Stability testing of HPCs and MNCs from apheresis products
WBC counts and flow cytometry markers remain stable for up to 3 days in samples collected from fresh HPC and MNC products when stored at 4 °C, although cell viability progressively declines. However, RNA-seq data reveal early transcriptomic changes in HPC samples, suggesting that immediate evaluation of these samples is critical to preserve their molecular integrity and functionality. These findings support the feasibility of delayed phenotypic analysis but emphasize the need for prompt molecular assays in HPC-based applications.
Authors
- Anh Dinh (ORCID: https://orcid.org/0009-0002-9715-7743)
- Lipei Shao (ORCID: https://orcid.org/0000-0003-3009-7096)
- Jinxia Ma (ORCID: https://orcid.org/0000-0003-4393-8720)
- Chunjie Jiang (ORCID: https://orcid.org/0000-0002-3033-092X)
- Kathryn R. Martin (ORCID: https://orcid.org/0000-0003-1053-069X)
- Tatyana Fuksenko
- David F. Stroncek (ORCID: https://orcid.org/0000-0001-5867-3265)
- Nan Zhang (ORCID: https://orcid.org/0000-0001-6518-2501)
- Hui Liu (ORCID: https://orcid.org/0000-0002-4568-8051)
- Ping Jin (ORCID: https://orcid.org/0009-0006-5774-3421)
- Yihua Cai (ORCID: https://orcid.org/0000-0002-3693-9533)
- Yong Soo Kim
- Larry Moses
- Robert Somerville (ORCID: https://orcid.org/0000-0002-3093-2533)
Institutions
- Cellular Therapeutics (United Kingdom) (GB)
- National Institutes of Health Clinical Center (US)
Publication Details
- Journal
- Immunobiology
- Published
- 2025-08-29
- DOI
- https://doi.org/10.1016/j.imbio.2025.153112
- Primary Topic
- Hematopoietic Stem Cell Transplantation
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health