Variovorax arabinosiphilus sp. nov., Variovorax flavidus sp. nov., Variovorax gracilis sp. nov., Variovorax brevis sp. nov., Variovorax jilinensis sp. nov., Variovorax davisae sp. nov., Variovorax saccharolyticus sp. nov. and Variovorax fucosicus sp.
Thirteen novel strains - J2L1-78<sup>T</sup>, J2P1-59<sup>T</sup>, J22R24<sup>T</sup>, J22R133<sup>T</sup>, J22P168<sup>T</sup>, J22P271<sup>T</sup>, J22R187<sup>T</sup>, J22R193<sup>T</sup>, J2L1-63, J2R1-6, J22G21, J22G47, and J31P216 - were isolated from the rhizosphere of 20-year-old ginseng in Jilin Province, P.R. China. These strains were Gram-stain-negative, aerobic and rod-shaped. Phylogenetic analysis based on the 16S rRNA gene sequences indicated their affiliation to the genus <i>Variovorax</i>, with 98.8-99.6% sequence similarity to <i>Variovorax ginsengisoli</i> Gsoil 3165<sup>T</sup>, <i>Variovorax ureilyticus</i> UCM-2<sup>T</sup> and <i>Variovorax humicola</i> UC38<sup>T</sup>. Phylogenomic analysis demonstrated their distinctiveness from closely related species. Average nucleotide identity (ANI) values among these strains confirm their representation of eight distinct species; additionally, ANI and digital DNA-DNA hybridization values between these strains and their closest relatives were below 92.5 and 54.1%, respectively. Eight representative strains contained C<sub>16 : 0</sub>, summed feature 3 (C<sub>16 : 1</sub> <i> ω</i>7<i>c</i> and/or C<sub>16 : 1</sub> <i> ω</i>6<i>c</i>) and C<sub>17 : 0</sub> cyclo as major fatty acids. Based on phenotypic, phylogenetic and genotypic data, these 13 strains comprise 8 novel species within the genus <i>Variovorax</i>, for which we propose the following names: <i>Variovorax arabinosiphilus</i> sp. nov. (J2L1-78<sup>T</sup>=CGMCC 1.60704<sup>T</sup>=KACC 23365<sup>T</sup>), <i>Variovorax flavidus</i> sp. nov. (J2P1-59<sup>T</sup>=CGMCC 1.60707<sup>T</sup>=KACC 23366<sup>T</sup>), <i>Variovorax gracilis</i> sp. nov. (J22R24<sup>T</sup>=CGMCC 1.61001<sup>T</sup>=KACC 23367<sup>T</sup>), <i>Variovorax brevis</i> sp. nov. (J22R133<sup>T</sup>=CGMCC 1.61263<sup>T</sup>=KACC 23368<sup>T</sup>), <i>Variovorax jilinensis</i> sp. nov. (J22P168<sup>T</sup>=CGMCC 1.64555<sup>T</sup>=KACC 23372<sup>T</sup>), <i>Variovorax davisae</i> sp. nov. (J22P271<sup>T</sup>=CGMCC 1.64593<sup>T</sup>=KACC 23373<sup>T</sup>), <i>Variovorax saccharolyticus</i> sp. nov. (J22R187<sup>T</sup>=CGMCC 1.64629<sup>T</sup>=KACC 23374<sup>T</sup>) and <i>Variovorax fucosicus</i> sp. nov. (J22R193<sup>T</sup>=CGMCC 1.64631<sup>T</sup>=KACC 23375<sup>T</sup>).
Authors
- Yu‐Hang Jiang
- Yu‐Hua Xin (ORCID: https://orcid.org/0000-0001-6534-5964)
- Leilei Yang (ORCID: https://orcid.org/0009-0007-4093-0332)
- Jian Ye (ORCID: https://orcid.org/0000-0002-7741-4354)
- Cece Yin (ORCID: https://orcid.org/0009-0005-3728-3926)
- Qing Liu (ORCID: https://orcid.org/0000-0003-2700-9956)
Institutions
- Guangxi University (CN)
- Chinese Academy of Sciences (CN)
- Vector & Vector-Borne Diseases Research Institute (TZ)
- Institute of Microbiology (CN)
- University of Chinese Academy of Sciences (CN)
Publication Details
- Journal
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
- Published
- 2025-08-29
- DOI
- https://doi.org/10.1099/ijsem.0.006895
- Citations
- 1
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Natural Science Foundation of China