Ultrasound assisted preparation of lignin-chitosan capped gold nanoparticles: Its evaluation for suzuki coupling and treatment of cervical cancer

As a natural capping, reducing, and stabilizing template, lignin-chitosan (Lig-CS) hydrogel polymers were created by hydrogen bonding and cross-linking with glutaraldehyde to immobilize gold (Au) nanoparticles (Lig-CS/Au NPs) upon exposure to ultrasonic radiation. The Lig-CS/Au NPs nanocomposite’s formation was verified using a number of methods, including X-ray diffraction (XRD), Energy Dispersive X-ray (EDX)-elemental mapping, Transmission Electron Microscopy (TEM), Inductively Coupled Plasma (ICP), UV-vis, and Field-Emission Scanning Electron Microscopes (FE-SEM). The particles were found to be round, monodispersed, and roughly 7.15 nm in size by TEM imaging. Lig-CS/Au NPs are pure crystalline, as indicated by the corresponding XRD pattern. Furthermore, the Lig-CS/Au NPs’ catalytic actions were used to create biphenyl derivatives using Suzuki-Miyaura coupling. The resultant nanocatalyst showed reusability for more than seven runs without experiencing a discernible drop in activity. The cytotoxicity and anti-cervical cancer activity of Lig-CS/Au NPs against the HeLa, SiHa, and CCI-PI 19 cell lines were evaluated using the MTT test kit to evaluate their biological impact. Accordingly, the corresponding IC 50 values were 454, 642, and 745 µg/mL. The cell viability decreased in a dose-dependent manner during treatment with the bio-nanomaterial. Lig-CS/Au NPs’ antioxidant activity was assessed using a DPPH test, which yielded an IC 50 value of 72 µg/mL.