FORMULATION AND EVALUATION OF ALOE VERA HYDRO GEL USING COTTON SEED OIL

The main objective of present work is to prepare and evaluate Aloe vera hydro gel using cotton seed oil and evaluate physical and microbial parameters for the formulated gel.Six different formulations were prepared using different concentrations of Sodium Carboxy methyl cellulose.After three days, the formulations were weighed, to check the percentage moisture loss for the gel and it was found to be less than 1gm.The pH of all formulations were checked initially after formulation and after 1 week.Viscosity also checked initially for all the formulations using Brookfield viscometer and found satisfactory for all formulations except Trial 1 and 2. Viscosity is an important feature to determine the resistance of flow of gel formulation so that it can spread on the skin properly.It was determined with the help of Brookfield viscometer using different spindles and concluded to use spindle number 2.Nutrient agar media was used for microbial growth study.Aloe Vera gel was aseptically transferred onto nutrient agar plates with blank and sample (n=2).Petri plates were used for microbial study and the gel samples were aseptically transferred on to the sample plates in a cross pattern.The inoculated plates were then incubated at optimal conditions for microbial growth.Daily microbial examination of the agar plates was checked and evaluated for a period of 14 days.Observations were checked for any visible growth, colony morphology and characteristics.