HR-LCMS PROFILING AND CYTOTOXICITY STUDY OF CRUDE AQUEOUS EXTRACT OF PHASEOLUS VULGARIS LINN. LEAVES

The objective of the current study is to evaluate the phytochemical profile and cytotoxic properties of the crude aqueous extract of Phaseolus vulgaris Linn.leaves.This plant is well-known for its nutritional and agricultural benefits, and its crude extract was obtained through the sequential extraction method.The extract was then subjected to High-Resolution Liquid Chromatography-Mass spectrometry (HR-LCMS) analysis to identify the phytoconstituents present.A total of 120 phytoconstituents were obtained, with 26 compounds being identified as major constituents in both positive and negative ESI modes.Sapropterin, Melibiose, Pirbuterol, Osmaronin, N-Isovalerylglycine methyl ester, Beta-D-Galactopyranosyl-(1->4) -beta-D-galactopyranosyl-(1->4) -D-galactose, 3-Methyladenine, 3-Beta, 6-Beta-Dihydroxynortropane, D-pipecolic acid, Lotaustralin, 8-Hydroxyadenine, L-threo-3-Phenylserine, L-isoleucyl-L-proline, Gentianadine, L-Arogenate, Prolyl-Arginine, Norcotinine, Ganolucidic acid E, Benzocaine, Succinoadenosine, D-Galactonate, Melibiose, Thiolactomycin, (Z)-5-[(5-Methyl-2-thienyl)methylene] -2(5H) Furanone, Rutin, and Kuwanon Z.The toxicity of crude extracts at various concentrations was