Dual-tracer autoradiographic analysis of glucose metabolism and hypoxia in orthotopic and PDX tumor models
Hypoxic sub-volumes (FAZA/pimonidazole) were commonly present. A reasonable spatial overlap between FAZA and 14C-2DG was observed in most lung and oropharyngeal tumors with R typically exceeding 0.55. In the breast tumor model, the extent of overlap between FAZA and 14C-2DG varied widely with R ranging from 0.03 to 0.82, which may relate to intertumor mutational differences in this Her2+ oncogene-driven model. Our results suggest a putative role for FDG-PET to identify hypoxic foci and guide dose-escalation.
Authors
- Martin F. Berthelsen
- Johan Bussink (ORCID: https://orcid.org/0000-0002-5751-4796)
- Morten Busk (ORCID: https://orcid.org/0000-0001-5711-1017)
- Martin Kristian Thomsen (ORCID: https://orcid.org/0000-0002-5055-7531)
- Jacob Kinggaard Lilja‐Fischer (ORCID: https://orcid.org/0000-0002-6400-6650)
- Mikkel Holm Vendelbo (ORCID: https://orcid.org/0000-0003-0431-2522)
- Henrik Hager (ORCID: https://orcid.org/0000-0002-3164-9190)
- Jens Overgaard (ORCID: https://orcid.org/0000-0002-0814-8179)
- Kim Vang Hansen (ORCID: https://orcid.org/0000-0002-5665-3284)
- Steen Jakobsen
- Ebbe Boedtkjers
Institutions
- Radboud University Nijmegen (NL)
- Aarhus University (DK)
- Aarhus University Hospital (DK)
- Radboud University Medical Center (NL)
Publication Details
- Journal
- Acta Oncologica
- Published
- 2025-08-30
- DOI
- https://doi.org/10.2340/1651-226x.2025.44002
- Primary Topic
- Cancer, Hypoxia, and Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 0.00