Evaluation and Comparison of Sirtuin 1 Levels in Serum of Periodontally Healthy and Diseased Subjects and Its Response to Nonsurgical Periodontal Treatment: A Clinico-biochemical Study

A BSTRACT Background: Sirtuin 1 (SIRT-1) regulates major metabolic processes including oxidative stress epigenetically reprograms inflammation. SIRT1 regulates human periodontal ligament cells and helps differentiate them into osteoblasts, which is an important step in periodontal bone regeneration. However, the impact of nonsurgical periodontal treatment on SIRT-1 levels remains unknown. Hence, the study aimed to evaluate and compare SIRT-1 levels in the serum of periodontally healthy and diseased subjects and its response to nonsurgical periodontal treatment. Materials and Methods: It was an experimental in vivo study. Fifty samples were included in the study using a simple random sampling method. In healthy (Group I) and periodontitis patients (Group II), the periodontal clinical parameters were recorded and serum sample collection was done at baseline. In the periodontitis group, the parameters were re-evaluated 1 month after scaling and root planing (Group II [X]). Levels of SIRT-1 were analyzed by enzyme-linked immunosorbent assay to evaluate the difference between the tree groups. Results: There was no difference in the level of SIRT-1 in Groups I and II. Furthermore, there was no change in their levels after scaling and root planing in the chronic periodontitis group. Conclusions: Within the limitations of the present study, no association was observed between the serum levels of SIRT-1 in healthy, pre- and postperiodontitis treatment. Hence, according to our research, SIRT-1 cannot be suitable as a biomarker for periodontal disease.