Spectral and Biofunctional Evaluation of Aniline-o-Toluidine Nanostructured Copolymer: Assessing the Cytotoxicity Activity on MG-63 Cancer Cell Lines

In this work, poly(aniline-co-o-toluidine) (POT) nanostructured porous copolymer was synthesized through chemical polymerization in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB). The obtained POT copolymers were investigated by FT-IR, XRD, UV and SEM-EDX techniques. The distinct antibacterial and antifungal activities have exhibited by the as-synthesized copolymer. Various bacterial strains such as Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae have possess better antimicrobial efficacy than polyaniline. Furthermore, the antifungal properties of POT have exhibited greater effectiveness than those of polyaniline against Candida albicans and Aspergillus niger strains. The antioxidant capacity has measured against DPPH radicals highlighted a stronger activity in POT copolymer, with IC50 values of 29.4 µg/mL for polyaniline and 41.02 µg/mL for POT copolymer. Additionally, the cytotoxicity has been performed on MG-63 cancer cell lines, which indicated an IC50 value of 58.95 µg/mL for POT copolymer. The obtained results revealed that the development of POT based copolymer that are promising candidates for various applications, including biomedical, genetic engineering, pharmaceutical, and drug delivery fields. The obtained POT copolymer exhibited as an excellent antibacterial and anticancer activity, suggesting its potential roles in tissue engineering scaffolds, anticancer treatments, and various biomedical applications.