ISOLATION AND IDENTIFICATION OF MULTI-DRUG RESISTANT STAPHYLOCOCCUS AUREUS FROM POST-OPERATIVE PUS SAMPLE

Staphylococcus aureus can cause a wide range of infections ranging from minor skin abscesses to more serious invasive diseases.The development and spread of bacterial strains that are resistant to antibacterial drugs has emerged as a global problem.The appearance of antibiotic resistant bacteria over the past decades has been regarded as an inevitable genetic response to the strong selective pressure imposed by antimicrobial chemotherapy, which plays a crucial role in the evolution of antibiotic resistant bacteria.The present study was aimed to isolate and determine the antibiotic susceptibility pattern of Staphylococcus aureus strain against some conventional and traditional antibiotics.Post surgical pus samples were collected from nearby Hospital and species identification was confirmed by Gram staining and standard biochemical tests.Antibiotic susceptibility tests were carried out by determination of Minimum inhibitory concentration, minimum bactericidal concentration, DAD test and BHI vancomycin screening agar test.From this study, it was observed that isolated S. aureus strains are pathogenic; 50% of strains were resistant to penicillin G, ampicillin and erythromycin; 40% strains were resistant to cephotaxime, gentamycin, streptomycin, tetracycline, chloramphenicol, norfloxacin, methicillin and vancomycin.Our study exposed the appearance of VRSA in Midnapore surroundings which is very alarming and may soon become a comprehensive problem, unless antimicrobial agents are used more wisely.