Analysis of Inositol Phosphates, Diphosphoinositol Phosphates, and Their Analogs by HPLC on an Acid-Compatible Stationary Phase with Detection by Complexation with Ferric Ion
High-performance anion-exchange chromatography with acid eluents allows for effective separation of myo-inositol tetrakisphosphates, myo-inositol pentakisphosphates, diphospho-myo-inositol phosphates (inositol pyrophosphates), inositol hexakisphosphates, inositol pyrophosphate analogs, and carbohydrate-based analogs of inositol phosphates. All are detectable by postcolumn addition of ferric ion. In the following, we provide examples drawn from characterization of ITPK, IPK1, and IP<sub>3</sub> 3K enzymes.
Authors
- Andrew M. Riley (ORCID: https://orcid.org/0000-0001-9003-3540)
- Charles A. Brearley (ORCID: https://orcid.org/0000-0001-6179-9109)
- Colleen Sprigg (ORCID: https://orcid.org/0000-0001-7755-4245)
- Megan L. Shipton (ORCID: https://orcid.org/0000-0002-9982-0927)
- Hayley Whitfield (ORCID: https://orcid.org/0000-0001-6003-7874)
- Barry V. L. Potter (ORCID: https://orcid.org/0000-0003-3255-9135)
Institutions
- University of East Anglia (GB)
- Norwich Research Park (GB)
- University of Oxford (GB)
Publication Details
- Journal
- Methods in molecular biology
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1007/978-1-0716-4799-8_6
- Primary Topic
- Phytase and its Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00