Acute Effects of Pelemir-Enriched Bread on Postprandial Glucose and Hormonal Responses in Adults with Obesity, Type 2 Diabetes, and Normal Weight: A Two-Phase Exploratory Study

<b>Background:</b> Pelemir (<i>Cephalaria syriaca</i>) is a bitter-tasting ancestral legume with a high polyphenol content and emerging potential as a functional food ingredient. This study investigated the acute metabolic effects of pelemir-enriched bread in adults. <b>Methods:</b> In this two-phase non-randomized trial, 60 participants in three groups (<i>n</i> = 20 per group: healthy controls [HCs], individuals with obesity [OB], and individuals with type 2 diabetes [T2D]) consumed regular or pelemir-enriched bread on two separate test days. Postprandial glucose, insulin, C-peptide, GLP-1, PYY, ghrelin, leptin, triglyceride, and IL-6 were measured over 120 min. Subjective appetite ratings were evaluated using visual analog scales (VASs). The incremental area under the curve (iAUC) values were compared using Wilcoxon tests and linear mixed-effects models. <b>Results:</b> Pelemir-enriched bread significantly increased iAUCs for insulin (<i>p</i> = 0.014), C-peptide (<i>p</i> = 0.046), and GLP-1 (<i>p</i> = 0.039) compared to regular bread. There was no significant change in iAUC for glucose. Group-stratified analyses showed a higher postprandial iAUC of glucose, insulin, and C-peptide in the OB group compared to the HC group. VAS-based appetite ratings did not show significant changes in hunger, fullness, or desire to eat, but a borderline significant reduction was observed in prospective food consumption after pelemir-enriched bread (<i>p</i> = 0.050). <b>Conclusions</b>: Acute consumption of pelemir-enriched bread may modulate postprandial insulin and incretin responses. Its modest impact on subjective appetite regulation supports further investigation of pelemir as a functional food rich in polyphenols, especially in populations with metabolic dysfunction.