Genome-Wide Identification and Characterization of BPC Transcription Factors in Tobacco (Nicotiana tabacum)

BASIC PENTACYSTEINE (BPC) is a small family of plant-specific transcription factors that play crucial roles in plant growth, development processes, and response to abiotic stresses. However, the specific roles of Nicotiana tabacum BPCs (NtBPCs) remain ambiguous. Here, we identified 12 NtBPC genes, 5 of which were mapped to four chromosomes. Phylogenetic analysis classified these genes into three subfamilies. Collinearity was observed among BPC genes of N. tabacum, Capsicum annuum, and Solanum lycopersicum. Moreover, polypeptides encoded by NtBPC genes within the same subfamily shared similar conserved motifs and protein domains. Subcellular localization showed that 10 NtBPC proteins are localized in the nucleus. Promoter analysis revealed the presence of abiotic stress response elements in the promoters of NtBPCs. Further tissue-specific expression analysis using RT-qPCR revealed that NtBPCs are highly expressed in stems and leaves. After drought, NaCl, and cold treatments, NtBPCs exhibited varied expression patterns. These findings provide valuable insights into the evolutionary dynamics of the NtBPC gene family and lay the groundwork for subsequent investigations into the functions of NtBPC genes.