Evaluation of methods for the measurement of antibody-dependent enhancement of dengue virus infection using different FcγRIIa expressing cell lines
In summary, infection-enhancement assay using K562 cells was superior to U937 and Vero-CD32a cells in evaluating ADE. Samples with high neutralizing activity demonstrated very low levels of infection-enhancing activity in Vero-CD32a cells. Comparison of FRNT titres in Vero and Vero-CD32a cells is not suitable for detecting ADE. Our findings suggest that infection-enhancing activities are apparent at sub-neutralizing concentrations of dengue virus antibodies in all individuals exposed to dengue virus.
Authors
- Vidya A. Arankalle (ORCID: https://orcid.org/0000-0002-9940-3437)
- Shubham Shrivastava (ORCID: https://orcid.org/0000-0002-5980-8536)
- Shweta Chelluboina
- Darshan Kshirsagar
- Akhilesh Chandra Mishra (ORCID: https://orcid.org/0000-0003-1836-1990)
- Gauri Panzade
Institutions
- Bharati Vidyapeeth (Deemed to be University) (IN)
Publication Details
- Journal
- PLoS ONE
- Published
- 2025-08-29
- DOI
- https://doi.org/10.1371/journal.pone.0331320
- Citations
- 1
- Primary Topic
- Mosquito-borne diseases and control
- Type
- article
- Field-Weighted Citation Impact
- 1.88
Funders
- Johns Hopkins University
- Department of Biotechnology, Ministry of Science and Technology, India
- National Institutes of Health
- Johns Hopkins Bloomberg School of Public Health
- Biotechnology Industry Research Assistance Council