Increased Expression of Bacterial Cellulose Synthase Genes in <i>Komagataeibacter Xylinus</i> Exposed to a Rotating Magnetic Field

The objective of this study was to investigate the alterations in the expression of <i>bacterial cellulose synthase</i> (<i>bcs</i>) genes in<i>Komagataeibacter xylinus</i>, dependent on the exposure duration and specific parameters of a rotating magnetic field (RMF).<i>K. xylinus</i> cells were subjected to an RMF at frequencies of 5 and 50 Hz for durations ranging from 12 to 72 h. Gene expression was assessed using quantitative polymerase chain reaction (qPCR). The findings demonstrated that RMF exposure significantly enhanced BC production efficiency. Specifically, the average yields of wet and dry BC following RMF treatment increased by 28% and 18%, respectively, compared to the control. These improvements correlated with upregulated <i>bcs</i> gene expression, which showed statistically significant changes at both frequencies and all time points. The strongest effects occurred at 5 Hz. In conclusion, RMFs represent a promising approach to modulate gene expression in bacteria, offering substantial biotechnological potential, particularly for enhancing BC production.