ERYTHROCYTE BLOOD INDICES IN RATS UNDER CONDITIONS OF ACETAMINOPHEN-INDUCED TOXIC INJURY AFTER PARTIAL HEPATECTOMY

The work focuses on investigating the erythrocyte indices of rat blood under conditions of acetaminophen-induced toxic injury following partial liver resection. Modeling of partial hepatectomy was performed using the method of Mitchell & Willenbring by surgically removing two-thirds of the liver. The determination of the erythrocyte count, their mean volume, hemoglobin concentration, its mean content, and mean concentration in erythrocytes was conducted at 24 (priming phase), 48 (active cell proliferation phase), 72 (termination phase), and 168 hours (distant period) after the performance of partial hepatectomy using an RT-7600 (Rayto) automatic hematology analyzer. It was established that after partial liver tissue resection in control rats, a decrease in hemoglobin levels and erythrocyte count occurs during the first three days, indicating the development of postoperative anemia, which is partially a physiological phenomenon. Instead, in animals with toxic injury, a decrease in hemoglobin concentration and a reduction in erythrocyte count take place throughout the entire regenerative period, with a deepening state of anemia and erythropenia during the termination phase (72 h) and the distant period (168 h). Liver tissue regeneration after partial hepatectomy in control rats is characterized by slight normochromia because of a decrease in the mean content and concentration of hemoglobin in erythrocytes at the early stages of regeneration, with a distant macrocytosis effect. Partial liver resection in animals with toxic injury is accompanied by the development of hypochromic microcytic anemia, with intensification during the terminal stages (72 h and 168 h) of the regenerative process. Keywords: hemoglobin, erythrocytes, acetaminophen, partial hepatectomy, regeneration