Morphological and phylogenetic analyses reveal two new species of Neohelicomyces (Tubeufiales, Tubeufiaceae) from China

<i>Neohelicomyces</i> is a genus of helicosporous hyphomycetes with the potential to produce bioactive secondary metabolites. During a survey of helicosporous fungi in Guizhou and Hainan provinces, southern China, four isolates were obtained from both freshwater and terrestrial habitats. Based on combined analyses of multigene phylogenetic data (ITS, LSU, <i>tef</i>1-α, and <i>rpb</i>2) and morphological characteristics, two novel species, <i>Neohelicomyces aquisubtropicus</i> and <i>N. wuzhishanensis</i>, are proposed. Detailed descriptions, illustrations, and phylogenetic analyses of the new taxa are presented. Additionally, a checklist of currently accepted <i>Neohelicomyces</i> species supported by molecular data is provided.