Photodynamic Therapy Using Pulsed-Laser Irradiation of Gold Nanoparticles for the Treatment of Cancers with High Basal Oxidative Stress
For over 20 years, considerable research has been carried out on the use of nanotechnologies in cancer treatment. Beyond the photothermal effect of irradiating gold nanoparticles, the photodynamic effect with reactive oxygen species (ROS) production at the tissue level is poorly known. We showed that renal cancers had high basal ROS levels. We engineered different gold nanoparticles and optimized the irradiation parameters. Then, we intravenously injected several patient-derived xenografts with tailored gold nanorods that we irradiated using a pulsed laser. We optimized irradiation parameters to decrease toxic effects on normal tissue and showed that the local monitoring of skin temperature was an accurate marker for predicting histological toxicity. We demonstrated a significant additional effect of irradiating gold nanoparticles, with the increased activation of the autophagy pathway following irradiation, associated with the disappearance of renal cancer cells. Our study highlights the elevated ROS levels in cancers as a potential therapeutic target for ROS-mediated cytotoxicity therapies.
Authors
- Benoît Caron (ORCID: https://orcid.org/0000-0001-7051-4339)
- Diaddin Hamdan (ORCID: https://orcid.org/0000-0002-2212-0287)
- Bruno Palpant (ORCID: https://orcid.org/0000-0003-1376-2533)
- Yoann Lalatonne (ORCID: https://orcid.org/0000-0003-2235-6561)
- Léa Tourneur (ORCID: https://orcid.org/0000-0001-6636-1982)
- Erwann Guénin (ORCID: https://orcid.org/0000-0002-7125-9074)
- Christophe Comte
- Christophe Lebœuf (ORCID: https://orcid.org/0000-0002-6537-0550)
- Guilhem Bousquet (ORCID: https://orcid.org/0000-0001-5594-6694)
- Sylvie Marguet (ORCID: https://orcid.org/0000-0002-8670-1320)
- Khanh‐Van Do
- E. Van Glabeke
- Syrine Gueffrache
- Justine Paris (ORCID: https://orcid.org/0000-0002-6260-2854)
- Alexandra Marsocci
Institutions
- Université de Technologie de Compiègne (FR)
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Université Paris Cité (FR)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- Université Paris-Saclay (FR)
- Sorbonne Université (FR)
- Université Sorbonne Paris Nord (FR)
- Institut des Sciences de la Terre de Paris (FR)
- CentraleSupélec (FR)
- Association pour la Recherche en Physiologie de l’Environnement (FR)
- CEA Paris-Saclay (FR)
- Hôpital Avicenne (FR)
- Laboratoire de Recherche Vasculaire Translationnelle (FR)
- Equipe de Recherche en Epidémiologie Nutritionnelle (FR)
- Hôpital La Porte Verte (FR)
- Université Paris 1 Panthéon-Sorbonne (FR)
Publication Details
- Journal
- Nano Letters
- Published
- 2025-08-29
- DOI
- https://doi.org/10.1021/acs.nanolett.5c03580
- Primary Topic
- Nanoplatforms for cancer theranostics
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Agence Nationale de la Recherche