Mucin 1 antigen mimetic functionalized mannosylated peptide nanofibers for antigen uptake and immune modulation
In this work, immune modulation of monocyte-derived dendritic cells was studied using <i>in vitro</i> cell culture and organ-on-chip models. Self-assembled mannosylated peptide nanofibers were prepared and integrated with the MUC1 TnThr mimetic Tn-MIM, to stimulate DCs' maturation and differentiation. The nanofibers obtained (namely Man-PA/Tn-MIM) showed remarkable cell-penetrating capabilities and allowed the correct presentation of the antigen mimetic with a corresponding induction of lymphocyte, iNKT and NK cell recruitment. The Man-PA/Tn-MIM integrated nanofibers were also found to direct the immune response towards the Th1 phenotype, which is responsible for the production of cellular and humoral immunity. Organ-on-chip tests confirmed the cytotoxicity of lymphocytes stimulated with DCs exposed to Man-PA/Tn-MIM. These results will help in the development of immunotherapies avoiding complications due to heterogeneous antigen presentation and the use of adjuvants.
Authors
- Silvia Scaglione (ORCID: https://orcid.org/0000-0002-9464-3554)
- Andrea Sodini (ORCID: https://orcid.org/0000-0002-0113-5797)
- Mustafa O. Güler (ORCID: https://orcid.org/0000-0003-1168-202X)
- Cristina Nativi (ORCID: https://orcid.org/0000-0002-6312-3230)
- Maria Elisabetta Federica Palamà (ORCID: https://orcid.org/0000-0001-5585-137X)
- Federica Cefalì (ORCID: https://orcid.org/0000-0003-0416-2130)
- Gaëtan Lesca (ORCID: https://orcid.org/0000-0001-7691-9492)
- Silvia Fallarini (ORCID: https://orcid.org/0000-0001-8871-7417)
- Ayşe B. Tekinay (ORCID: https://orcid.org/0000-0002-4453-814X)
- Francesca Maestrelli (ORCID: https://orcid.org/0000-0002-7732-7381)
- Filippo Susini
- Maria Cristina Salvatici
Institutions
- Università degli Studi del Piemonte Orientale “Amedeo Avogadro” (IT)
- University of Chicago (US)
- International Medical Research (Germany) (DE)
- Institute for the Chemistry of OrganoMetallic Compounds (IT)
- Ostim Technical University (TR)
- University of Florence (IT)
- University of Genoa (IT)
Publication Details
- Journal
- Biomaterials Science
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5bm00304k
- Primary Topic
- Supramolecular Self-Assembly in Materials
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Banca d'Italia
- Associazione Italiana per la Ricerca sul Cancro