Single-cell profiling delineates the tumor microenvironment and immunological networks in patient-derived uterine leiomyosarcoma
For the first time, a comprehensive single-cell map of ULSA was constructed, depicting a metastasis-susceptible cell subset (U11-EDARADD) and an extremely immunosuppressed tumor microenvironment dominated by depleted CD8<sup>+</sup>T cells, M2 macrophages and N2 neutrophils. These features shed light on the underlying mechanisms of chemotherapy resistance and immunotherapy failure. The biomarkers identified here (EDARADD, CLDN10, TMIGD2) as well as the dysregulated pathways (TGF-β, angiogenesis, MIF signaling) provide possible targets for future development of combined immunotherapy strategies against this deadly disease.
Authors
- Meiyi Chen (ORCID: https://orcid.org/0000-0003-1761-9232)
- Chenghao Wu (ORCID: https://orcid.org/0000-0001-5449-9765)
- Yuhang Xiao (ORCID: https://orcid.org/0000-0001-6406-6664)
- Dongsheng Shen (ORCID: https://orcid.org/0000-0002-0872-835X)
- Huaifang Li (ORCID: https://orcid.org/0000-0002-9496-7145)
- Xiaowen Tong (ORCID: https://orcid.org/0000-0003-4872-1678)
- Fang Li (ORCID: https://orcid.org/0000-0003-0342-3941)
- Yi Guo (ORCID: https://orcid.org/0000-0002-3369-5385)
- Rujun Chen (ORCID: https://orcid.org/0000-0002-9451-6280)
- Lina Yang (ORCID: https://orcid.org/0000-0002-7414-5910)
Institutions
- Tongji University (CN)
- Shanghai Medical College of Fudan University (CN)
- Fudan University (CN)
- Fudan University Shanghai Cancer Center (CN)
- International Peace Maternity & Child Health Hospital (CN)
- Shanghai East Hospital (CN)
- Obstetrics and Gynecology Hospital of Fudan University (CN)
- Tongji Hospital (CN)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-08-29
- DOI
- https://doi.org/10.3389/fimmu.2025.1653096
- Citations
- 1
- Primary Topic
- Immune cells in cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.80