Alternate dyes for image-based profiling assays
Substituting MitoBrilliant or Phenovue phalloidin 400LS for standard mitochondrial or actin dyes minimally impacted Cell Painting assay performance. Phenovue phalloidin 400LS offers the advantage of isolating actin features from Golgi or plasma membrane while accommodating an additional 568 nm dye. Live cell imaging, enabled by ChromaLive dye, provides real-time assessment of compound-induced morphological changes. Combining this with the standard Cell Painting assay significantly expands the feature space for enhanced cellular profiling. Our findings provide data-driven options for researchers selecting dye sets for image-based profiling.
Authors
- Patrick J. Byrne (ORCID: https://orcid.org/0000-0003-2569-6019)
- John Arévalo (ORCID: https://orcid.org/0000-0002-1138-5036)
- Suganya Sivagurunathan (ORCID: https://orcid.org/0000-0002-9778-5400)
- Beth A. Cimini (ORCID: https://orcid.org/0000-0001-9640-9318)
- Anne E. Carpenter (ORCID: https://orcid.org/0000-0003-1555-8261)
- Alán F Muñoz (ORCID: https://orcid.org/0000-0001-8698-5477)
- Shantanu Singh (ORCID: https://orcid.org/0000-0003-3150-3025)
- Maria Kost‐Alimova (ORCID: https://orcid.org/0000-0002-5257-4909)
Institutions
- Broad Institute (US)
Publication Details
- Journal
- SLAS DISCOVERY
- Published
- 2025-08-28
- DOI
- https://doi.org/10.1016/j.slasd.2025.100268
- Citations
- 3
- Primary Topic
- Cell Image Analysis Techniques
- Type
- article
- Field-Weighted Citation Impact
- 4.90
Funders
- Foundation for the National Institutes of Health
- Eli and Edythe Broad Foundation
- Brigham and Women's Hospital
- Broad Institute
- Chan Zuckerberg Initiative
- National Institutes of Health