Green rapid HPLC method for testing retinol and tocopherol in ophthalmic gels

• The suggested technique, is a unique one which uses cheap, and simple elution to quantify vitamin A and vitamin E in pure and eye gel is straightforward and environmentally friendly. • Several assessment indicators were used to quantitatively evaluate the approaches' sustainability and greenness. • Examining the blueness and whiteness characteristics was another goal of the recently introduced White Analytical Chemistry (WAC). • The suggested approach was validated and found to be in compliance with US FDA and ICH regulations. In addition to providing immediate comfort for dry and irritated eyes, vitamin A (retinol) and E (tocopherol) drops strengthen the eyes' defenses. Optic gels, which are compatible with contact lenses, use vitamins A and E to improve vision, soothe and hydrate dry and sensitive eyes, and provide immediate comfort. It is now feasible to determine the amounts of vitamin A and vitamin E in ocular gels thanks to the development and validation of a thorough, rapid, simple, and eco-friendly stability-indicating LC approach. In compliance with ICH guidelines, the two medications underwent a series of assessments. Vitamin A palmitate, vitamin E acetate, and related degradants were successfully resolved via isocratic separation. Utilizing the octadecylsilyl phase at ambient temperature (25° C ± 5° C), both drugs were quantified and qualified. Constant ratios of filtered water (neutral purified water at pH range 5 to 7) to ethanol comprise the isocratic mobile phase, which has a flow rate of 2.0 mL/min. During the 290 nm detection, the concentrations of vitamin A palmitate and vitamin E acetate varied from 5 to 200 IU/mL and 25 to 1000 μg/mL, respectively. The LC technique's ecological benefits are demonstrated by its overall AES grade of 79, AGREE grade of 0.64, MoGAPI grade of 76, BAGI grade of 80, and final whiteness of 95.8. According to research, the suggested method is accurate, reliable, long-lasting, eco-friendly, and distinctive.