An alginate-bacitracin conjugate based multifunctional electrospinning nanofiber dressing for infected wound healing

• An alginate-bacitracin conjugate (SA-BAC) was prepared by condensation reaction. • Multi-functional electrostatically spun nanofiber dressing containing SA-BAC (PSA-BAC) prepared by electrostatic spinning machine. • Validation of adhesion, moisture retention, biocompatibility, mechanical strength, thermodynamic stability antimicrobial properties and cytotoxicityof a multifunctional electrostatically spun nanofiber dressing (PSA-BAC). • The multifunctional electrostatically spun nanofiber dressing PSA-BAC has a desirable wound healing effect in the S. aureus infected wound model. About half of community-acquired wound infections are infections caused by S. aureus . Infected wounds are often difficult to heal and cause a range of adverse reactions. Consequently, healing of infected wounds remains an urgent challenge in modern medicine. Conventional wound dressings for healing often exhibit low adhesion, poor moisture retention, and inadequate antimicrobial properties, all of which hinder effective wound healing. To address these shortcomings, sodium alginate-bacitracin (SA-BAC) was aimed to be synthesized by grafting sodium alginate (SA) and bacitracin (BAC) in this study. A multifunctional electrostatically spun nanofiber dressing (PSA-BAC) containing SA-BAC and polyvinyl alcohol (PVA) was developed. PSA, PBAC, and PSA + BAC dressings were prepared as control dressings. Compared with the control group, PSA-BAC dressing has better physicochemical properties and biocompatibility than the control dressing especially with good adhesion and high water solubility. In addition, in vivo experiments using animal skin injury models infected with bacteria revealed that the PSA-BAC dressing provided excellent antibacterial properties. Analysis of tissue regeneration and expression of relevant inflammatory factors in animal wounds indicated that the PSA-BAC dressing exerted a healing effect on the infected wounds. Further research and improvement of this dressing may yield even better outcomes in the treatment of bacteria-infected wounds.