Deep tissue profiling of Populus stem at single nucleus level reveals uncharacterized cell types and cell-specific gene regulatory networks
Our study demonstrates the capture and transcriptome characterization of cell types embedded in plant secondary growth, identifying novel regulators of xylem development and stress adaptation. The discovery of MYB48 as a key regulator of VAC function highlights a previously uncharacterized mechanism influencing vessel development, with applications to improving wood formation and stress resilience.
Authors
- Daniel Conde (ORCID: https://orcid.org/0000-0001-8362-4190)
- Kelly M. Balmant (ORCID: https://orcid.org/0000-0001-5189-5121)
- Paolo M. Triozzi (ORCID: https://orcid.org/0000-0003-0192-3336)
- Wendell J. Pereira (ORCID: https://orcid.org/0000-0003-1019-6281)
- Matias Kirst (ORCID: https://orcid.org/0000-0002-8186-3945)
- Henry W. Schmidt (ORCID: https://orcid.org/0000-0003-3917-3219)
- Christopher Dervinis (ORCID: https://orcid.org/0000-0002-2176-4232)
Institutions
- Scuola Superiore Sant'Anna (IT)
- University of Florida (US)
- Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (ES)
Publication Details
- Journal
- Genome biology
- Published
- 2025-08-28
- DOI
- https://doi.org/10.1186/s13059-025-03728-x
- Citations
- 2
- Primary Topic
- Plant Gene Expression Analysis
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- U.S. Department of Energy
- Office of Science