Development of a selective-iodide indicator for live-cell imaging and evaluation of CFTR activity
Cystic fibrosis (CF) arises from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Monitoring I<sup>-</sup> transport serves as a critical approach for evaluating CFTR function in live cells, providing a foundation for the development of diagnostic tools and therapeutic treatments. Here, we report an iridium(iii) complex (<b>I-Sense</b>) for the selective and pH-independent imaging of intracellular I<sup>-</sup>. By tracking cellular iodide I<sup>-</sup> uptake, <b>I-Sense</b> facilitates the evaluation of CFTR activity in live cells, providing a valuable tool for the functional characterization of CFTR activity.
Authors
- Ka‐Ho Leung (ORCID: https://orcid.org/0000-0002-2998-669X)
- Gabriella Ballestas (ORCID: https://orcid.org/0000-0002-4498-3980)
- Jared Morse
- Prasanna Ganesh
- Fung Kit Tang
- Kathrine Cowart
Institutions
- University of South Carolina (US)
- Clarkson University (US)
Publication Details
- Journal
- Sensors & Diagnostics
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5sd00086f
- Primary Topic
- Neonatal Respiratory Health Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Clarkson University
- St. Lawrence University
- National Institutes of Health
- National Institute of General Medical Sciences
- Division of Materials Research