Qualitative analysis, total quantification of secondary metabolites & their characterization by TLC profile in the Leaf and Bark of Ricinus communis L.

The particular phytocompound performs a relevant physiological mechanism in humans. Methanolic crude form of extract of Ricinus communis Linn. is a well-known herbal plant, which was analysed by qualitative and quantitative estimations. The analysis of total quantitative estimations of specific phytochemical constituents from Ricinus communis L. includes Alkaloids, Flavonoids, Phenols, Tannins & Steroids. Total average appropriate phenolic compounds were found in significantly larger amounts, especially in leaves & bark, which goes along with tannin concentration, alkaloid, steroids, & flavonoids. The maximum content of total phenolics (3.42 ± 0.8 Mg FCAE/gram DW) in leaves & (15.39 ± 2.32 Mg FCAE/gram DW) in bark, tannin (3.24 ± 0.95 Mg GAE/gram DW) in leaves & (8.3 ±0.051 Mg GAE/gram DW). A considerable quantity of total alkaloids was observed (2.50 ± 1.06 Mg AE/gram of DW in the leaf & (3.54 ± 0.67 Mg AE/gram DW) in bark, which followed the total concentration of steroid (2.34 ± 0.1 Mg FDE/gram DW) in leaves & (4.41 ± 2.64 Mg FDE/gram DW) in bark. Total concentration of flavonoid (1.85 ± 0.7 Mg QE/gram DW) in leaves & (6.8 ± 0.02 Mg QE /gram DW) in bark of Ricinus communis L. Phytochemical assessment was carried out through a range of qualitative approaches, and TLC studies were done with multiple solvent systems of varying polarity. Qualitative phytochemical determination by chemical tests indicates the presence of alkaloids, steroids, saponins, tannins, anthocyanins, coumarins, flavonoids, reducing sugars, glycosides, and phenols in plants. TLC profiling of these plants was carried out using different solvents in methanol extracts of Ricinus communis L., and it showed Distinct Rf values. Different solvent systems were observed under UV light to detect phytochemicals present in the extracts. (A) CHCL3, C6H14, C2H5OH (4:2:4) B) CHCL3, C6H14, H2O, PE, C4H8O2 (4:2:2:4) (C) PE, CHCL3, C6H14 (4:3:3).