Intracellular and Extracellular Vesicle <scp>miRNA</scp> Signatures in Human <scp>iPSC</scp> ‐Derived Neural Stem Cells and Floor Plate Progenitors
Refined control of intrinsic and extrinsic signals is critical for specific neuronal differentiation. Here, we differentiated human induced pluripotent stem cells (hiPSCs) from three different healthy donors into neural stem cells (NSCs) and floor plate progenitors (FPPs; progenitors of dopaminergic neurons) and further performed intracellular and extracellular vesicles' (EVs) miRNA profiling. While NSC and FPP cells differed significantly in levels of only 8 intracellular miRNAs, their differences were more evident in the EV miRNAs with 27 differentially expressed miRNAs. Target validation of intracellular miRNAs revealed that FPPs expressed more EXOC5 mRNA than NSCs, which is implicated in the function of primary cilia, an essential signaling organelle in FPPs. Moreover, we found a group of 5 miRNAs consistently enriched in EVs from these three cell types. This study presents a foundation for the field of miRNA regulation in neural development and provides new insights for disease modeling and regenerative medicine.
Authors
- Camila M. Lopes‐Ramos (ORCID: https://orcid.org/0000-0003-0284-7371)
- Zhiyun Wei (ORCID: https://orcid.org/0000-0002-3554-4142)
- Frederico Moraes Ferreira (ORCID: https://orcid.org/0000-0002-7403-9165)
- Hyder A. Jinnah (ORCID: https://orcid.org/0000-0003-1531-3969)
- Marilene H. Lopes (ORCID: https://orcid.org/0000-0003-2496-0674)
- William T. Hendriks
- Anna M. Krichevsky (ORCID: https://orcid.org/0000-0002-5080-5507)
- Lilian Cruz
- Xandra O. Breakefield
- D. Cristopher Bragg
- Helder I. Nakaya
Institutions
- Brigham and Women's Hospital (US)
- Harvard University (US)
- Emory University (US)
- Universidade de São Paulo (BR)
- Hospital Israelita Albert Einstein (BR)
- Massachusetts General Hospital (US)
- Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (BR)
- National Institute of Science and Technology (ID)
Publication Details
- Journal
- The FASEB Journal
- Published
- 2025-08-28
- DOI
- https://doi.org/10.1096/fj.202501157r
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- American Lung Association
- Susan and Leonard Feinstein Inflammatory Bowel Disease Clinical Center at Mount Sinai
- Boston Area Diabetes Endocrinology Research Center
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- National Institutes of Health
- National Heart, Lung, and Blood Institute
- National Cancer Institute
- National Institute of Neurological Disorders and Stroke