Monitoring Wolfberry (Lycium barbarum L.) Canopy Nitrogen Content with Hyperspectral Reflectance: Integrating Spectral Transformations and Multivariate Regression

Accurate monitoring of canopy nitrogen content in wolfberry (Lycium barbarum L.) is essential for optimizing fertilization management, improving crop yield, and promoting sustainable agriculture. However, the sparse, architecturally complex canopy of this perennial shrub—featuring coexisting branches, leaves, flowers, and fruits across maturity stages—poses significant challenges for canopy spectral-based nitrogen assessment. This study integrates methods across canopy spectral acquisition, transformation, feature spectral selection, and model construction, and specifically explores the potential of hyperspectral remote sensing, integrated with spectral mathematical transformations and machine learning algorithms, for predicting canopy nitrogen content in wolfberry. The overarching goal is to establish a feasible technical framework and predictive model for monitoring canopy nitrogen in wolfberry. In this study, canopy spectral measurements are systematically collected from densely overlapping leaf regions within the east, south, west, and north orientations of the wolfberry canopy. Spectral data undergo mathematical transformation using first-derivative (FD) and continuum-removal (CR) techniques. Optimal spectral variables are identified through correlation analysis combined with Recursive Feature Elimination (RFE). Subsequently, predictive models are constructed using five machine learning algorithms and three linear regression methods. Key results demonstrate that (1) FD and CR transformations enhance the correlation with nitrogen content (max correlation coefficient (r) = −0.577 and 0.522, respectively; p < 0.01), surpassing original spectra (OS, −0.411), while concurrently improving model predictive capability. Validation tests yield maximum R2 values of 0.712 (FD) and 0.521 (CR) versus 0.407 for OS, confirming FD’s superior performance enhancement. (2) Nonlinear machine learning models, by capturing complex canopy-light interactions, outperform linear methods and exhibit superior predictive performance, achieving R2 values ranging from 0.768 to 0.976 in the training set—significantly outperforming linear regression models (R2 = 0.107–0.669). (3) The Random Forest (RF) model trained on FD-processed spectra achieves the highest accuracy, with R2 values of 0.914 (training set) and 0.712 (validation set), along with an RPD of 1.772. This study demonstrates the efficacy of spectral transformations and nonlinear regression methods in enhancing nitrogen content estimation. It establishes the first effective field monitoring strategy and optimal predictive model for canopy nitrogen content in wolfberry.