Mettl15-Mettl17 modulates the transition from early to late pre-mitoribosome
The biogenesis of the mitoribosomal small subunit involves a dynamic network of assembly factors. Conserved methyltransferases Mettl15 and Mettl17 act on the solvent-exposed surface of rRNA. Binding of Mettl17 is associated with the early assembly stage, whereas Mettl15 is involved in the late stage. Here, we integrate structural data from Trypanosoma brucei with mammalian homologs and molecular dynamics simulations. We reveal how the interplay of Mettl15 and Mettl17 in intermediate steps links the distinct stages of small subunit assembly. The analysis suggests a model wherein Mettl17 acts as a platform for Mettl15 recruitment. Subsequent release of Mettl17 allows a conformational change of Mettl15 for substrate recognition. Upon methylation, Mettl15 adopts a loosely bound state which leads to its replacement by initiation factors, concluding the assembly. Together, our results indicate that assembly factors Mettl15 and Mettl17 cooperate to regulate the biogenesis process.
Authors
- Tomáš Pánek (ORCID: https://orcid.org/0000-0002-1738-8430)
- Ondřej Gahura (ORCID: https://orcid.org/0000-0002-2925-4763)
- Alena Zı́ková (ORCID: https://orcid.org/0000-0002-8686-0225)
- Björn Nystedt (ORCID: https://orcid.org/0000-0001-7809-7664)
- Claudio Mirabello (ORCID: https://orcid.org/0000-0001-7868-034X)
- Yury Zgadzay (ORCID: https://orcid.org/0000-0002-0801-4656)
- Paul C. Whitford (ORCID: https://orcid.org/0000-0001-7104-2265)
- Alexey Amunts (ORCID: https://orcid.org/0000-0002-5302-1740)
- Prashant Chauhan (ORCID: https://orcid.org/0000-0001-7752-5380)
- George Wanes
Institutions
- Linköping University (SE)
- Uppsala University (SE)
- Charles University (CZ)
- Science for Life Laboratory (SE)
- Institut de génétique et de biologie moléculaire et cellulaire (FR)
Publication Details
- Journal
- Structure
- Published
- 2025-08-28
- DOI
- https://doi.org/10.1016/j.str.2025.08.002
- Citations
- 1
- Primary Topic
- Microtubule and mitosis dynamics
- Type
- article
- Field-Weighted Citation Impact
- 1.15
Funders
- National Science Foundation
- European Commission
- Ministerstvo Školství, Mládeže a Tělovýchovy
- Grantová Agentura České Republiky
- Science for Life Laboratory
- National Institutes of Health
- HORIZON EUROPE Framework Programme
- European Research Council