Septal LYVE1 <sup>+</sup> macrophages control adipocyte stem cell adipogenic potential
Tissue macrophages reside in anatomically distinct subtissular niches that shape their identity and function. In white adipose tissue (WAT), we identified three macrophage populations with distinct localization, turnover, and phenotypes. Septal adipose tissue macrophages (sATMs), marked by CD209b and lymphatic vessel endothelial hyaluronan receptor 1, were long-lived and positioned in close proximity to adipocyte stem cells (ASCs) within the WAT septum. Within this shared niche, sATMs instructed the differentiation of ASCs into white adipocytes through transforming growth factor-β1 (TGFβ1). Depletion of sATMs, or the selective loss of TGFβ1 within tissue-resident macrophages, redirected ASC fate toward thermogenic adipocytes, enhancing WAT beiging and protecting against diet-induced obesity. These findings highlight the role of a discrete, anatomically defined macrophage population that governs ASC fate and orchestrates adipose tissue expansion.
Authors
- Hwee Ying Lim (ORCID: https://orcid.org/0000-0003-3991-7855)
- Josephine Lum (ORCID: https://orcid.org/0000-0003-1108-5989)
- Sue‐Anne Toh (ORCID: https://orcid.org/0000-0003-1570-4417)
- Nicolas Venteclef (ORCID: https://orcid.org/0000-0002-6313-1214)
- Peter See (ORCID: https://orcid.org/0000-0003-4428-7763)
- Diego Adhemar Jaitin (ORCID: https://orcid.org/0000-0003-0166-1575)
- Kenji Kabashima (ORCID: https://orcid.org/0000-0002-0773-0554)
- Bing Su (ORCID: https://orcid.org/0000-0003-0871-7666)
- David Alejandro Bejarano (ORCID: https://orcid.org/0000-0001-7804-0131)
- Zhaoyuan Liu (ORCID: https://orcid.org/0000-0002-3117-0833)
- Camille Blériot (ORCID: https://orcid.org/0000-0002-4298-2888)
- Wan Ting Kong (ORCID: https://orcid.org/0000-0002-9271-841X)
- Katharine Yang (ORCID: https://orcid.org/0000-0002-8209-1289)
- Lai Guan Ng (ORCID: https://orcid.org/0000-0003-1905-3586)
- Mathilde Bied
- Florent Ginhoux (ORCID: https://orcid.org/0000-0002-2857-7755)
- Xiaotong Yu
- Ido Amit (ORCID: https://orcid.org/0000-0003-2968-877X)
- Benoît Malleret (ORCID: https://orcid.org/0000-0001-9658-7528)
- Zachary Chow (ORCID: https://orcid.org/0000-0003-4493-8730)
- Honghao Liu (ORCID: https://orcid.org/0000-0002-7722-2547)
- Shu Wen Tan
- J. Xu (ORCID: https://orcid.org/0009-0009-8214-9418)
- Véronique Angeli (ORCID: https://orcid.org/0000-0002-2759-3497)
- Svetoslav Chakarov (ORCID: https://orcid.org/0000-0002-0665-5417)
- Yuin‐Han Loh (ORCID: https://orcid.org/0000-0002-4715-6454)
- Lingling Sheng (ORCID: https://orcid.org/0000-0002-5056-167X)
- Junwen Qu (ORCID: https://orcid.org/0000-0001-6146-4720)
- Andreas Schlitzer (ORCID: https://orcid.org/0000-0001-7662-3712)
- Sonia Baig (ORCID: https://orcid.org/0000-0001-5195-3512)
- Lucie Orliaguet (ORCID: https://orcid.org/0000-0002-3209-457X)
- Yanan Hu
- Gowshika Rengasamy
- Xiaomeng Zhang (ORCID: https://orcid.org/0000-0002-3794-6823)
- Ziyi Li (ORCID: https://orcid.org/0000-0003-0947-9137)
- Christopher Lee (ORCID: https://orcid.org/0000-0001-9802-4550)
- Jing Tian
- Xiujun Fu (ORCID: https://orcid.org/0009-0000-6656-1837)
- Danning Zheng (ORCID: https://orcid.org/0000-0002-1056-7161)
- Jinmiao Chen (ORCID: https://orcid.org/0000-0001-7547-6423)
- M. Shabeer Yassin (ORCID: https://orcid.org/0009-0003-1514-9377)
Institutions
- Agency for Science, Technology and Research (SG)
- University of Bonn (DE)
- Central South University (CN)
- National University of Singapore (SG)
- Shanghai Jiao Tong University (CN)
- Université Paris Cité (FR)
- Aix-Marseille Université (FR)
- Kyoto University (JP)
- Renji Hospital (CN)
- Singapore Immunology Network (SG)
- Institut Gustave Roussy (FR)
- Institut Necker Enfants Malades (FR)
- Shanghai Ninth People's Hospital (CN)
- SingHealth Duke-NUS Academic Medical Centre (SG)
- Centre d’Immunologie de Marseille-Luminy (FR)
- Xiangya Hospital Central South University (CN)
- A*STAR Graduate Academy (SG)
- Weizmann Institute of Science (IL)
- Institute of Molecular and Cell Biology (SG)
Publication Details
- Journal
- Science
- Published
- 2025-08-28
- DOI
- https://doi.org/10.1126/science.adg1128
- Citations
- 16
- Primary Topic
- Adipose Tissue and Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 18.63