Hyperspectral Imaging for Distinguishing Granulomatous Mastitis From Normal Breast Tissue in Core Needle Biopsies
This study explores the use of hyperspectral imaging (HSI) to distinguish granulomatous mastitis (GM) from normal breast tissue in core-needle biopsy specimens. High-resolution spectral data were captured from paraffin-embedded sections across the 400-1000 nm range. Following preprocessing, normalization, and principal component analysis, one-way analysis of variance revealed ten wavelengths with the greatest diagnostic power. Notably, hemoglobin absorption peaks and lipid-collagen signatures provided the strongest spectral discrimination between GM and healthy tissue. These results underscore HSI's promise as a rapid, real-time adjunct for preoperative and intraoperative evaluation of breast lesions.
Authors
- H.‐J. Lv (ORCID: https://orcid.org/0000-0002-1371-1376)
- Yukun Yin
- Chanchan Zhu
- Cuilei Wei
- Jingwei Li (ORCID: https://orcid.org/0000-0002-6129-2751)
- Mengdi Zhang (ORCID: https://orcid.org/0000-0002-3239-4804)
- Minmin Yu (ORCID: https://orcid.org/0000-0003-3385-4603)
- Mengqiu Zhang (ORCID: https://orcid.org/0000-0003-1662-4785)
- Guangshang Zhong (ORCID: https://orcid.org/0009-0001-4704-5762)
- Xinqian Dong
Institutions
- Shandong University of Traditional Chinese Medicine (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- National Cancer Center (US)
- Affiliated Hospital of Shandong University of Traditional Chinese Medicine (CN)
Publication Details
- Journal
- Journal of Biophotonics
- Published
- 2025-08-27
- DOI
- https://doi.org/10.1002/jbio.202500240
- Primary Topic
- Optical Imaging and Spectroscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Natural Science Foundation of Shandong Province