Establishing an <i>ex vivo</i> porcine skin model to investigate the effects of broad-spectrum antiseptic on viable skin microbial communities
Broad-spectrum antiseptics are widely used to prevent surgical site infection and as wound cleansing agents. The impacts of such agents on beneficial microbes in the skin microbiome are understudied. Here, we describe an <i>ex vivo</i> skin model to test the impacts of antiseptics or other topical agents on the healthy skin microbiome.
Authors
- Shelby Sandstrom (ORCID: https://orcid.org/0000-0001-9813-5875)
- Jennifer J. Meudt
- Anna Huttenlocher A (ORCID: https://orcid.org/0000-0001-7940-6254)
- Lindsay Kalan (ORCID: https://orcid.org/0000-0003-4980-5128)
- Angela Gibson (ORCID: https://orcid.org/0000-0003-1181-7481)
- Lusheng Huang (ORCID: https://orcid.org/0000-0002-6940-667X)
- Dhanansayan Shanmuganayagam (ORCID: https://orcid.org/0000-0002-8882-1668)
- Elizabeth C. Townsend (ORCID: https://orcid.org/0000-0003-3102-8033)
- Kui Xu (ORCID: https://orcid.org/0000-0001-6931-3070)
- Karinda De La Cruz
Institutions
- University of Wisconsin–Madison (US)
- Discovery Centre (CA)
- McMaster University (CA)
Publication Details
- Journal
- mSphere
- Published
- 2025-08-28
- DOI
- https://doi.org/10.1128/msphere.00441-25
- Citations
- 2
- Primary Topic
- Bacterial Identification and Susceptibility Testing
- Type
- article
- Field-Weighted Citation Impact
- 2.64
Funders
- National Institutes of Health