Synergistic Role of Trichoderma viride with Other Phosphate Solubilizing Microorganisms for Enhancing Phosphate Solubilization in Wheat Cultivation of Manipur

Background: The study investigates the role of Trichoderma viride (T.V) and other phosphate solubilizing microorganisms (PSM) in enhancing phosphorus solubilisation and other nutrient availability in wheat (Triticum aestivum) cultivation. Methods: The present work was conducted at FEEDS experimental farm, Manipur on 15/12/ 2021 in Wheat (Shriram super 252). The experiment was carried out with six treatments(T), T1 @ Trichoderma viride (T.V), T2 @ Azotobacter chroococcum (A.C) + Trichoderma viride (T.V), T3 @ Pseudomonas putida (P.P) + Trichoderma viride (T.V), T4 @ Azotobacter chroococcum (A.C) + Pseudomonas putida (P.P) + Trichoderma viride, T5 @ Azotobacter chroococcum (A.C) and T6 @ Azotobacter chrocooccum (A.C) + Pseudomonas putida (P.P) with four replications. Graded doses of nitrogen (N), phosphorus (P) and potassium (K) are applied in the ratio 100:50:50. Soil sample for PSM enumeration were collected at 0,30,65 and 95 days after sowing (DAS). Result: The results demonstrated a significant increase in microbial populations, phosphorus solubilisation, nutrient availability and grain yield. Out of the six treatments, T3 which received T. V and P. P showed highest Carbon (c), P and Potassium(K) content and T6 showed highest Nitrogen(N) content in the soil. The mean of the highest total Phosphate solubilizing bacteria (PSB) population is observed in 30 DAS in T6 and the lowest total PSB population in T1 at 65 DAS. The mean of the highest total Phosphate solubilizing fungi (PSF) population is at 30 DAS in T1 and Lowest PSF population detected is in T6 at 0 DAS. The highest yield was observed in T4@ Azotobacter chroococcum (A.C) + Pseudomonas putida (P.P) + Trichoderma viride indicating a synergistic effect.