Comparative Analysis of Plant Defense Activation by Four Biosurfactants: Mode of Action and Disease Control Potential

Grapevine (<i>Vitis vinifera</i>) is highly susceptible to fungal diseases, particularly downy mildew caused by <i>Plasmopara viticola</i>. Environmental contamination and potential health risks to viticulturists have raised concerns about the long-term sustainability of chemical control. In this study, we evaluated the potential of four biosurfactants-surfactin, rhamnolipid, sophorolipid, and spiculisporic acid-as alternative agents to chemical fungicides for disease management in viticulture. Surfactin, rhamnolipid, and sophorolipid, but not spiculisporic acid, significantly reduced the severity of grape downy mildew and strawberry anthracnose and induced the expression of defense-related genes, such as β-1,3-glucanase and class IV chitinase, in grapevine and strawberry leaves, although each biosurfactant triggered distinct gene expression patterns. Utilizing salicylic acid (SA)- and jasmonate (JA)-insensitive mutants of <i>Arabidopsis thaliana</i>, we found that sophorolipid induced plant resistance through the canonical SA signaling pathway. In contrast, plant resistance induced by surfactin and rhamnolipid was independent of both the SA and JA signaling pathways. Notably, sophorolipid was the only biosurfactant that induced systemic acquired resistance in grapevine leaves through unknown signaling pathways, suppressing <i>P. viticola</i> infection at sites distant from the treatment area. These findings suggest that biosurfactants, particularly sophorolipids, are a promising eco-friendly alternative to conventional fungicides in viticulture.