Electrochemical simulation of psychotropic drug metabolism compared to in vivo processes using liquid chromatography and mass spectrometry

Data from <i>in vivo</i> experiments agreed with the data from electrochemical oxidation, which predicted some of the potential metabolites found in the human liver microsomes. EC-(LC)-MS is well-suited for the simulation of the oxidative metabolism of selected psychotropic drugs and acts as the orthogonal source of information about drug metabolites compared to liver microsomes and biological matrices. EC-(LC)-MS enables the direct identification of reactive TPs, circumvents time-consuming sample preparation and is ethically advantageous because it reduces the need for animal experiments.