Janus Hydrogel Microparticles with Tea Polyphenol/Melanin Nanoparticles Integration from Microfluidics for Wound Healing

Massive bioactives show outstanding performance in promoting wound healing, while their combined application remains challenging in avoiding mutual interference. Herein, Janus hydrogel microparticles co-loaded with tea polyphenol-magnesium and melanin nanoparticles for synergistically antioxidant and antibacterial wound treatment are proposed. The tea polyphenol-magnesium nanoparticles are prepared by the self-assembly of tea polyphenol molecule and magnesium ion, while melanin nanoparticles are harvested from black hair. These two kinds of nanoparticles are, respectively, embedded into the hemisphere of Janus microparticles by microfluidics technology. The in vitro tests reveal that the resultant Janus microparticles have high biocompatibility and show enhanced antioxidant performance and synergistical antibacterial activity. Additionally, when the Janus microparticles are applied to the treatment of diabetic wounds, they promote the wound healing by scavenging reactive oxygen species, killing bacteria, reducing inflammation, decreasing matrix metalloproteinase-9 secretion, and enhancing tissue remolding. Therefore, it is believed that the Janus microparticles with melanin and tea polyphenol-Mg nanoparticles are highly promising candidate for clinical wound healing.