Analyte-Induced Restriction of Intramolecular Rotation: Turn-On aza-BODIPY Sensor for Biothiol Detection in Aqueous Media
Two alternate aza-BODIPY fluorophores bearing a formyl-functional group were synthesized and evaluated for biothiol sensing. Both provide high binding constants (up to 9800 M<sup>-1</sup>) and low limits of detection (as low as 1 μM). More importantly, this study introduces an unconventional "turn-on" sensing mechanism, in which fluorescence enhancement arises from analyte-induced restriction of intramolecular rotation rather than analyte-triggered cleavage of quencher. This alternative strategy provides a promising platform for the development of next-generation fluorescent probes.
Authors
- Ayşe Daut Özdemir (ORCID: https://orcid.org/0000-0001-8547-523X)
- Bleda Can Sadıkoğulları (ORCID: https://orcid.org/0000-0003-1633-2993)
Institutions
- Division of Chemistry (US)
- Istanbul Technical University (TR)
Publication Details
- Journal
- Organic Letters
- Published
- 2025-08-27
- DOI
- https://doi.org/10.1021/acs.orglett.5c02543
- Citations
- 2
- Primary Topic
- Sulfur Compounds in Biology
- Type
- article
- Field-Weighted Citation Impact
- 2.22