The Effect of Fagonia cretica on Inflammation Induced by Hyperhomocysteinemia and Antioxidant Activities: HPLC Analysis of the Butanolic Compound

In the present study the phenolics of F. cretica extract was identified by HPLC, which revealed the presence of 3,4,5-Trimethoxycinnamic acid - 3,5-Dimethoxyphenol- 4- Ethylphenol- 4-Methylguaiacol- 3,5-Dihydroxybenzoic acid- Coniferyl alcohol- 3- Hydroxybenzoic acid- 2,4-Dihydroxybenzoic acid. The total flavonol, flavonoid and phenolis contents of the F. cretica extract were appreciated. Additionally, the antioxidant activity of the extract was checked. The protective effect of F. cretica on mice cardivacular inflammation induced by hyperhomocysteinemia was evaluated according to an experimental in vivo procedure of 21 days. The level of plasma inflammatory markers (hs-CRP, homocysteine), were measured. Additionally, the histological sections of the liver, heart and aorta tissues were examined. Our results revealed a potent antioxidant capacity of F. cretica extract. On the other hand, F. cretica considerably reduced the histopathological changes, decreased the levels of Hcy and hs-CRP, Therefore, we recommend the use of F. cretica as a natural anti hyperhomocysteinemia and anti-inflammatory therapeutic agent.