First Detection of Encarsia smithi in Italy and Co-Occurrence with Eretmocerus iulii: A Case of Unintentional Introductions and New Associations with the Invasive Species Aleurocanthus spiniferus

The occurrence of the invasive Orange Spiny Whitefly (<i>Aleurocanthus spiniferus</i>) has expanded rapidly in Italy, prompting responses from both native and unintentionally introduced natural enemies. Through field monitoring, morpho-molecular identification, and phenological analysis, a multi-species parasitoid complex acting on this pest was revealed. In addition to the predominant activity of <i>Eretmocerus iulii</i>, this study reports the first European detection of <i>Encarsia smithi</i>, genetically confirmed as belonging to haplogroup I, a lineage previously associated with <i>A. spiniferus</i> in Asia. Occasional parasitization by <i>Cales noacki</i> was also observed. Seasonal patterns suggest a possible ecological complementarity between <i>Er. iulii</i> and <i>E. smithi</i>, which may contribute to the suppression of <i>A. spiniferus</i> populations. Although climatic variables showed weak correlations with infestation or parasitism rates, a slight positive relationship was observed between rainfall and whitefly abundance. The increasing biocenotic complexity, reflected by the diversity and interactions among parasitoid species, indicates an emerging ecological balance. These findings underscore the potential role of unintentional biological control in supporting agroecosystem resilience.