Medium throughput protein expression and purification for protein design using heat lysis v1
This protocols describes medium throughput protein expression in E. coli using heat-lysis as a built in purification step. It is intended for preparation of heat-stable de novo designed proteins for screening in vitro.1-3 It provides a small but relatively pure lysate sample of the protein within 2 working days.
Authors
- Magnus Kjærgaard (ORCID: https://orcid.org/0000-0002-7020-9366)
- Francisca Pinheiro
- Vili Lampinen
- Ida K. Grene
Publication Details
- Published
- 2025-05-07
- DOI
- https://doi.org/10.17504/protocols.io.dm6gpq6b5lzp/v1
- Citations
- 1
- Primary Topic
- Viral Infectious Diseases and Gene Expression in Insects
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Research Foundation
- Danmarks Grundforskningsfond
- H. Lundbeck A/S