CAPG enhances adipogenesis and inflammatory cytokine expression in adipocytes

The expression of CAPG (capping actin protein, gelsolin-like) is upregulated in visceral white adipose tissue of high-fat diet -fed mice; however, its impact on adipocyte functionality remains unclear. We observed upregulated CAPG expression in the epididymal adipose tissue of high-fat diet-fed mice. To investigate the impact of CAPG on adipocyte differentiation and function, we generated a <i>Capg</i> knockdown 3T3-L1 cell line and induced adipogenic differentiation to mature adipocytes. Adipogenesis was assessed via Oil Red O and BODIPY staining, revealing that <i>Capg</i> knockdown markedly suppressed adipogenesis. Western blot analysis demonstrated that CAPG depletion reduced PPARγ expression. Additionally, Western blot analysis revealed that <i>Capg</i> knockdown significantly enhanced lipid utilization in adipocytes. ELISA and qPCR results further demonstrated that <i>Capg</i> knockdown effectively attenuated inflammatory responses in adipocytes. In conclusion, CAPG promotes adipogenesis and inflammatory responses, suggesting that targeted inhibition of CAPG may represent a potential therapeutic strategy for obesity-associated adipose tissue dysfunction.