Engineering prime editors in <i>Salvia miltiorrhiza</i> for precise genome modification
An optimized prime editing system in the model medicinal plant Danshen (Salvia miltiorrhiza) was developed, enabling base transversions, complex insertions and base substitutions. Precise editing of key genes generated germplasm with elevated contents of the bioactive abietane diterpene tanshinone, advancing synthetic biology applications in medicinal plants.
Authors
- Yifu Tian (ORCID: https://orcid.org/0000-0002-8167-5151)
- Han Zheng (ORCID: https://orcid.org/0000-0003-4692-7058)
- Muyao Yu (ORCID: https://orcid.org/0009-0009-8854-0725)
- Qi Liu
- Luqi Huang (ORCID: https://orcid.org/0000-0002-2070-4318)
- Qi Yao (ORCID: https://orcid.org/0000-0002-9813-4650)
- Yi Ye
Institutions
- Beijing University of Chinese Medicine (CN)
- Shanghai Jiao Tong University (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- China Academy of Chinese Medical Sciences (CN)
Publication Details
- Journal
- Journal of Integrative Plant Biology
- Published
- 2025-08-26
- DOI
- https://doi.org/10.1111/jipb.70006
- Citations
- 2
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- National Natural Science Foundation of China
- China Academy of Chinese Medical Sciences
- National Key Research and Development Program of China