Improved detection of isoniazid-heteroresistant <i>Mycobacterium tuberculosis</i> subpopulations by droplet digital PCR compared to MeltPro TB assay
Tuberculosis has emerged as a significant threat to global health, bringing numerous new cases and a large number of deaths annually. Isoniazid (INH), as a first-line treatment drug, plays a crucial role. However, the emergence of its drug resistance poses a tough challenge for tuberculosis control. Currently, the methods for detecting INH resistance and heteroresistance have limitations like the slow speed of traditional culture tests and the insufficient sensitivity of molecular methods. Therefore, improving the diagnostic efficacy of the detection results for INH resistance is crucial for the treatment of tuberculosis.
Authors
- Yu Pang (ORCID: https://orcid.org/0000-0001-6803-9807)
- Yuanyuan Shang (ORCID: https://orcid.org/0000-0002-3538-7730)
- Chianru Tan (ORCID: https://orcid.org/0000-0002-9032-6746)
- Yong Guo (ORCID: https://orcid.org/0000-0003-3175-3008)
- Songnan Wen (ORCID: https://orcid.org/0000-0002-6256-3189)
- Haoran Li (ORCID: https://orcid.org/0000-0001-5108-9218)
- Nan Wang (ORCID: https://orcid.org/0000-0003-0330-8636)
- Yilin Wang (ORCID: https://orcid.org/0000-0003-2503-8235)
- Mengjie Yang
Institutions
- Beijing Chest Hospital (CN)
- Tsinghua University (CN)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-08-26
- DOI
- https://doi.org/10.1128/spectrum.00030-25
- Primary Topic
- Tuberculosis Research and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Beijing Municipal Science and Technology Commission
- Beijing Nova Program