Examination of Alginite Mineral Supplementation During Fermentation of Probiotics and Its Effect on Skincare Activity of Ferment Lysates

Technological advancements, shifting consumer preferences, and societal changes drive the cosmetics industry to evolve continuously. The cosmetics industry is experiencing a renaissance, with new ingredients that are more environmentally friendly, natural, and transparent in terms of sourcing and manufacturing and, last but not least, which are also multifunctional. The use of technology in cosmetics has been rising, including AI (artificial intelligence) and AR (augmented reality) for virtual try-ons, skin analysis tools, and smart beauty devices that provide at-home skincare treatments. Meanwhile, fermented cosmetic ingredients are becoming increasingly popular in the beauty industry due to their improved efficacy and skin benefits. The benefits include enhanced absorption, improved stability (due to the self-produced preservatives), microbiome-friendliness (supporting the skin’s microbiome), and anti-inflammatory and soothing effects. The most common cosmetic ingredients produced by microorganisms are fermented rice, soy, green tea, fruits, and vegetables. Our laboratory investigates a mineral rock called alginite, which has shown many benefits in other fields, such as agriculture and cosmetics (e.g., as a facemask). It has been proven that alginite combined with LAB (lactic acid-producing bacteria) probiotics is beneficial for health and can increase biomass production. However, cell lysates with alginite have never been investigated for cosmetic purposes. This study aimed to investigate the potential of alginite, a mineral rock, in enhancing the cosmetic properties of LAB lysates, specifically focusing on antioxidant effects, skin-whitening properties, and, in preliminary tests, skin-moisturising effects. LAB strains were cultured with and without alginite, and the resulting cell lysates were analysed for these cosmetic applications. The preliminary results suggested that alginite may boost the hydrating effect of LAB lysate, increasing it tenfold compared to LAB lysate alone. The antioxidant effect was enhanced fivefold in the case of Lactobacillus acidophilus when cultured with alginite. However, no significant effect was observed on mushroom tyrosinase inhibition, suggesting no impact on pigment formation. Further research is needed to fully understand the mechanisms underlying these effects and to explore potential applications in cosmetic formulations. Limitations of this study include the focus on specific LAB strains and the need for in vivo studies to confirm the observed effects on human skin.