Reverse transcription recombinase polymerase amplification-lateral flow assay for detection of pathogenic orthoflaviviruses in mosquito vectors
This is the first study to develop an RPA-based method for the detection of multiple orthoflavivirus pathogens in mosquito vectors. The reverse transcription recombinase polymerase amplification assays with lateral flow dipsticks (RT-RPA-LFD) platform offers a rapid, cost-effective tool for identifying regions at risk of arboviral transmission, supporting the targeting of individual viral diseases. This technique holds promise as an early warning system for emerging arboviral threats in public health.
Authors
- Ronald Enrique Morales Vargas (ORCID: https://orcid.org/0000-0002-8013-5372)
- Werasak Surareungchai (ORCID: https://orcid.org/0000-0002-3639-7279)
- Patsamon Rijiravanich (ORCID: https://orcid.org/0000-0002-7080-9513)
- Parinda Thayanukul (ORCID: https://orcid.org/0000-0002-2903-0321)
- Pattamaporn Kittayapong (ORCID: https://orcid.org/0000-0002-4234-1239)
- N.D.A.D. Wijegunawardana (ORCID: https://orcid.org/0000-0001-6142-8375)
- Konkamon Sujijun
- Pimchanok Khumpeera
- Kittiya Suksawat
Institutions
- National Science and Technology Development Agency (TH)
- Mahidol University (TH)
- King Mongkut's University of Technology Thonburi (TH)
- Rajarata University of Sri Lanka (LK)
Publication Details
- Journal
- PeerJ
- Published
- 2025-08-26
- DOI
- https://doi.org/10.7717/peerj.19820
- Primary Topic
- Mosquito-borne diseases and control
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Mahidol University
- Faculty of Science, Mahidol University