Construction of a portable multiplex detection system for four bee viral paralysis diseases based on RT-PCR-microfluidic chip integrated technology
This system features simultaneous multi-target detection, high specificity, rapid processing, minimal instrumentation requirements, portability, and field applicability. It provides a robust tool for precise diagnosis and control of bee paralysis diseases, particularly suitable for resource-limited apiaries and outbreak scenarios, demonstrating significant practical value for safeguarding apicultural health.
Authors
- Dongliang Fei (ORCID: https://orcid.org/0000-0001-8583-9590)
- Yuming Liu
- Xinhua Shu (ORCID: https://orcid.org/0000-0003-3760-3019)
- Zhifeng Gao (ORCID: https://orcid.org/0000-0003-2984-8278)
- Ziyan Wang (ORCID: https://orcid.org/0000-0001-9264-2841)
- Mingxiao Ma
- Boyang Xia
- Tiantian Fei
Institutions
- Shenyang Center for Disease Control and Prevention (CN)
- Liaoning Jianzhu Vocational University (CN)
- First Affiliated Hospital of Henan University (CN)
- Jinzhou Medical University (CN)
Publication Details
- Journal
- BMC Veterinary Research
- Published
- 2025-08-26
- DOI
- https://doi.org/10.1186/s12917-025-04969-5
- Primary Topic
- Insect and Pesticide Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Key Research and Development Program of China