Semi-Targeted Nuclear Magnetic Resonance Metabolomics via Parahydrogen-Induced Hyperpolarization for Enhanced Sensitivity to Metabolic Composition

Non-hydrogenative para-hydrogen-induced polarization (nhPHIP) has proven a powerful tool for the enhanced NMR detection of several classes of metabolites in complex mixtures. Particularly, compounds carrying an α-amino acid motif have been previously detected and quantified in biological samples and natural extracts at submicromolar concentrations using 2D nhPHIP NMR spectroscopy. This technique is here applied for the first time in a semi-targeted metabolomics NMR study on urine from patients suffering from Pyridoxine-Dependent Epilepsy (PDE), currently diagnosed by the presence of dilute unique biomarkers. The signal enhancement, combined with the resolving power of 2D nhPHIP, results in a superior sensitivity to the metabolic composition compared to conventional <sup>1</sup>H NMR, demonstrating its potential in NMR-based metabolomics.

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Publication Details

Journal
Journal of the American Chemical Society
Published
2025-08-26
DOI
https://doi.org/10.1021/jacs.5c11226
Citations
4
Primary Topic
Advanced NMR Techniques and Applications
Type
article
Field-Weighted Citation Impact
3.03

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article

Semi-Targeted Nuclear Magnetic Resonance Metabolomics via Parahydrogen-Induced Hyperpolarization for Enhanced Sensitivity to Metabolic Composition

Thomas J. Boltje, Marco Tessari, Jonathan Martens, Ron A. Wevers et al.
4 citations
Journal of the American Chemical Society
Advanced NMR Techniques and Applications
3.03
article

Semi-Targeted Nuclear Magnetic Resonance Metabolomics via Parahydrogen-Induced Hyperpolarization for Enhanced Sensitivity to Metabolic Composition

Thomas J. Boltje, Marco Tessari, Jonathan Martens, Ron A. Wevers, Udo F. H. Engelke, Floris P. J. T. Rutjes, Jona Merx, Martin C. Feiters, Ruud L. E. G. Aspers, Thom B. Posthumus
article en
4 citations

Abstract

Non-hydrogenative para-hydrogen-induced polarization (nhPHIP) has proven a powerful tool for the enhanced NMR detection of several classes of metabolites in complex mixtures. Particularly, compounds carrying an α-amino acid motif have been previously detected and quantified in biological samples and natural extracts at submicromolar concentrations using 2D nhPHIP NMR spectroscopy. This technique is here applied for the first time in a semi-targeted metabolomics NMR study on urine from patients suffering from Pyridoxine-Dependent Epilepsy (PDE), currently diagnosed by the presence of dilute unique biomarkers. The signal enhancement, combined with the resolving power of 2D nhPHIP, results in a superior sensitivity to the metabolic composition compared to conventional <sup>1</sup>H NMR, demonstrating its potential in NMR-based metabolomics.

Journal of the American Chemical SocietyVol. 147(36)
Radboud University Nijmegen (NL), Radboud University Medical Center (NL)
Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Openalex Percentile: Top 8%
Advanced NMR Techniques and Applications
3.03
DOIOpen Access PDFOpenAlex
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Semi-Targeted Nuclear Magnetic Resonance Metabolomics via Parahydrogen-Induced Hyperpolarization for Enhanced Sensitivity to Metabolic Composition — Thomas J. Boltje, Marco Tessari, et al. · Journal of the American Chemical Society (2025) | TGRS Research Map | TGRS