A rapid and accurate method for Helicobacter pylori detection via integrating LAMP assay with CRISPR/Cas12b detection by one-step in one-pot
The integrated LAMP-CRISPR/Cas12b platform represents a promising alternative for the rapid and sensitive detection of HP. It combines the simplicity and rapidity of LAMP with the specificity of CRISPR/Cas12b, offering a robust, cost-effective, and high\-sensitivity diagnostic tool without the need for complex instrumentation. The method shows great potential for use in point-of-care testing (POCT) and could significantly enhance clinical practice by facilitating timely diagnosis and treatment of HP-related diseases.
Authors
- Yujing Guan
- Bo Ni (ORCID: https://orcid.org/0000-0003-2548-2539)
- Puhua Zhang (ORCID: https://orcid.org/0000-0001-9950-8258)
- Juping Gu (ORCID: https://orcid.org/0000-0003-0078-4602)
- Xingang Wang (ORCID: https://orcid.org/0000-0001-6641-8459)
- Yeqian Zhang
- Hui Cao (ORCID: https://orcid.org/0000-0003-3406-3803)
- Long Bai (ORCID: https://orcid.org/0000-0002-2026-5212)
- Xiang Xia (ORCID: https://orcid.org/0000-0002-8476-7916)
- Tao Liu (ORCID: https://orcid.org/0000-0003-1573-6777)
- Wei Sun (ORCID: https://orcid.org/0000-0002-3749-8717)
Institutions
- Second Military Medical University (CN)
- Renji Hospital (CN)
- Changhai Hospital (CN)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2025-08-26
- DOI
- https://doi.org/10.3389/fcimb.2025.1611134
- Citations
- 2
- Primary Topic
- Helicobacter pylori-related gastroenterology studies
- Type
- article
- Field-Weighted Citation Impact
- 4.01
Funders
- National Natural Science Foundation of China
- Renji Hospital