Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction
We successfully obtained an AXL monoclonal antibody only binding to Gas6/AXL complex. Using the antibody, we firstly found that AXL expressed on human pDCs had bind to Gas6. Not only applicated in flow cytometry, this novel antibody could also be used as capture antibody in ELISA. Depend on this antibody, we firstly discovered that sAXL Gas6 complex was significantly increased in patients with AMI. Compared to AXL detected by commercial kit, AXL-Gas6 complex we measured emerged as a more sensitive biomarker and risk factor for patients with ST-segment elevation myocardial infarction.
Authors
- Shouli Wang (ORCID: https://orcid.org/0000-0001-7958-1757)
- Zhaoliang Shan
- Tianlun Li
- Liguo Zhang (ORCID: https://orcid.org/0000-0002-2705-1399)
- Xuyuan Zhang (ORCID: https://orcid.org/0000-0002-6305-1556)
- Xiaohua Nie
- Bei Zhao
- Yin Zhao
Institutions
- Chinese Academy of Sciences (CN)
- Chinese PLA General Hospital (CN)
- Institute of Biophysics (CN)
- PLA Academy of Military Science (CN)
Publication Details
- Journal
- Frontiers in Medicine
- Published
- 2025-08-26
- DOI
- https://doi.org/10.3389/fmed.2025.1653708
- Primary Topic
- Phagocytosis and Immune Regulation
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Guangzhou Medical University
- National Natural Science Foundation of China
- Beijing Institute of Technology