High-throughput screening of monoclonal antibodies against carbapenemases using a multiplex protein microarray platform
Our findings confirm that protein-based microarrays offer a robust, efficient platform for antibody pair selection, reducing reagent use while accelerating assay development. The validated antibody pairs are directly applicable to ELISA or lateral flow test formats and provide a strong foundation for next-generation diagnostics capable of detecting an evolving panel of carbapenemases in clinical settings.
Authors
- Hugo M. Arends
- Celia Diezel
- Katrin Frankenfeld (ORCID: https://orcid.org/0000-0002-6761-5312)
- Martin Reinicke
- Stefan Monecke (ORCID: https://orcid.org/0000-0003-4368-8709)
- Thomas Schumacher (ORCID: https://orcid.org/0000-0003-0118-9119)
- Ralf Ehricht (ORCID: https://orcid.org/0000-0002-6612-0043)
- Elke Müller
- Sascha D. Braun
Institutions
- Leibniz Institute of Photonic Technology (DE)
- Research Centre of Medical Technology and Biotechnology (DE)
- InfectoGnostics Research Campus Jena (DE)
- Institut Virion\Serion (DE)
- Friedrich Schiller University Jena (DE)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2025-08-26
- DOI
- https://doi.org/10.3389/fmicb.2025.1650094
- Citations
- 1
- Primary Topic
- Advanced Biosensing Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Bundesministerium für Bildung und Forschung