In Situ Ionically Cross-Linkable Benzaldehyde-Modified Alginate and Its Bilayer Structure as a Tissue Sealant for Dural Closure
Effective tissue adhesives are vital for surgical applications. We synthesized <i>p</i>-aminobenzaldehyde-modified alginate (AL-ABA) with controlled degrees of substitution (DS, 3.8-13.7%) and cross-linked it ionically using CaCl<sub>2</sub>. AL-ABA solutions (3 w/v %) gelled rapidly (3-50 s), similar to unmodified alginate. Increasing DS reduced hydrogel stiffness but enhanced dura adhesiveness via Schiff's base formation with tissue amines, reaching 32 kPa at 9% DS. To optimize both adhesion and mechanical integrity, bilayer hydrogels were developed using an AL-ABA primer and AL cover layer, seamlessly bonded by ionic cross-linking. These AL-ABA/AL hydrogels showed strong dural adhesion and high burst pressure resistance (up to 406 mmHg). AL-ABA retained a low cytotoxicity and hemocompatibility. Ionically cross-linked AL-ABA and its bilayer configuration offer promising potential as biocompatible and efficient tissue sealants.
Authors
- Satoshi Fukushima (ORCID: https://orcid.org/0000-0002-0622-7682)
- Taichi Ito (ORCID: https://orcid.org/0000-0002-1589-8242)
- I‐Hsuan Yang (ORCID: https://orcid.org/0000-0002-9886-3044)
- Norimasa Iwasaki (ORCID: https://orcid.org/0000-0002-5496-4046)
- Seiichi Ohta (ORCID: https://orcid.org/0000-0002-4775-8199)
- Kazuki Matsumiya (ORCID: https://orcid.org/0009-0005-7937-3719)
- Mitsuko Isaji
- Arvind K. Singh Chandel (ORCID: https://orcid.org/0000-0002-7130-7734)
- Natsuko F. Inagaki (ORCID: https://orcid.org/0000-0002-6309-9023)
- Masahiko Takahata (ORCID: https://orcid.org/0000-0002-2436-2175)
- Kotaro Onishi
- Satoshi Shimizu (ORCID: https://orcid.org/0000-0002-7270-0781)
- Mineo Matsumoto (ORCID: https://orcid.org/0009-0009-5431-7920)
- Atsushi Mizushima (ORCID: https://orcid.org/0000-0002-1256-1919)
- Mai Yamada (ORCID: https://orcid.org/0000-0002-5891-8354)
- Yizhou Dai
- T. Iwabuchi
Institutions
- Mochida Pharmaceutical (Japan) (JP)
- Hokkaido University (JP)
- The University of Tokyo (JP)
Publication Details
- Journal
- Biomacromolecules
- Published
- 2025-08-25
- DOI
- https://doi.org/10.1021/acs.biomac.5c00748
- Citations
- 1
- Primary Topic
- Surgical Sutures and Adhesives
- Type
- article
- Field-Weighted Citation Impact
- 2.01
Funders
- Japan Agency for Medical Research and Development
- Mochida Pharmaceutical Company
- National Science and Technology Council